Raibat looking into the microscope in lab

Raibat Sarker ‘28

(he/him)

Home Department: Chemistry (Davis Lab)

Research Proejct: Understanding ultrafast peptide dynamics in cells using Fast Relaxation Imaging

My current research revolves around studying the folding dynamics of fast-folding peptides in cells. For this, I use Fast Relaxation Imaging (FReI), which combines fluorescence microscopy with laser induced temperature jumps. Since traditional FReI microscopes are not temporally fast enough to capture the sub-millisecond kinetics of fast folding peptides, I am working on improving the temporal resolution of our FReI microscope and use it to quantify the in cellulo thermodynamics and kinetics of a fast-folding peptide model, the WW domain proteins. My measurements will help validate in silico predictions and thus bridge the gap between experiments and simulations.

Relevant Publications

Knab and Davis (2023) Chemical interactions modulate λ6‐85 stability in cells

Time-resolved spectral imaging can be used to study protein folding in vitro, in single cells, and in whole organisms

Fast Relaxation Imaging (FReI) allows investigation of stability and dynamics in vitro and in living cells and organisms. Experimental investigations are compared to simulations to study cell function