Experimental realizations of volumetric/axially-evolving speckles with customized intensity probability density functions (PDFs). The intensity PDF is customized to be uniform within the range 0 ≤ I/⟨I⟩ ≤ 2 in (a) and to vary axially in (b) for two axial-decorrelation lengths (R_l). The speckle pattern evolves axially, regardless of whether the statistics is preserved or varied.
SeungYun Han ‘26
(he/him)
Home Department: Applied Physics (Cao Lab)
Research Project: Dynamic speckle illumination microscopy with tailored speckle statistics
Laser speckles have been widely used as illumination patterns for biomedical imaging. My research aims to enhance the imaging performance by tailoring the speckle statistics. Recently, I demonstrated three-dimensional customization of speckle statistics. One application that can be leveraged by this advancement is dynamic speckle illumination (DSI) microscopy, a fluorescent microscopy technique that uses random speckle patterns for wide-field illumination to achieve axial sectioning. I apply the tailored speckles to DSI and show significant improvements in both axial sectioning and imaging speed. With the axial sectioning ability surpassing confocal microscopy and no raster scanning, my approach provides a useful tool for dynamic imaging of live cells with higher speed and better axial resolution.
Relevant Publications
Han, Bender, and Cao. (2023) “Tailoring 3D speckle statistics”
